Super-Resolution Imaging
  | | | | | |
  DeltaVision|OMXTM

 

3D-SIMTM Super-Resolution Imaging

 

The DeltaVision|OMX system is based directly on the design and configuration of the OMX system developed and used at UCSF and licensed exclusively to Applied Precision. We have utilized our engineering and integration expertise to improve the optical configuration and electronic components. The system software was also redeveloped and now runs under Windows with greatly enhanced functionality and stability. Offering resolution at twice the diffraction limit of traditional microscopes, the DeltaVision|OMX system the practical solution for three dimensional super resolution imaging that does not depend on special fluorophores or fluorescent proteins.

  • Three Dimensional-Structured Illumination Microscopy (3D-SIM) Imaging
    - X,Y resolution between 80-120 nm
    - Z resolution between 250-350 nm (depending on wavelength)
    - >10 micron depth imaging (depending on spherical correction)
  • Widefield illumination light imaging path for fast conventional imaging
  • Simultaneous, multichannel imaging on up to 4 cameras
  • Standard configuration: 100x, 1.4 NA, objective lens, 2 x EM-CCD cameras, 405 nm and 488 nm lasers, personalDV workstation


(first image pair) Two isoforms of beta-tubulin in a cultured neuron - Images courtesy of Stefanie Kaech Petrie and Aurelie Snyder, Advanced Light Microscopy Core at The Jungers Institute Oregon Health & Sciences University. (second image pair) Hippocampal neurons - Images courtesy of Eric Dent, University of Wisconsin Madison

Download a brochure
Adobe PDF document, 771KB

Download spec sheet
Adobe PDF document, 128KB




 
     
  DeltaVision® is a family of fully integrated, optimized microscopy systems designed to look at multiple probes and samples over longer periods of time than other imaging systems. These systems are uniquely suited for 3D and 2D live cell applications. As biological questions increase in complexity and sensitivity, there is a greater need for higher image quality. Improved resolution and contrast yield greater quantitative measurements and therefore more reliable results.
    © 2010 Applied Precision, Inc.